Gavin Tiernan secured GB&I’s only singles victory with a 2&1 win over Michael la Sasso.
GB&I’s Connor Graham managed half point against fellow 18-year-old Mason Howell.
“Obviously we come here to win and obviously everyone is a little disappointed, but I think we can look back knowing that we all gave it 100%, and I think that’s all that really matters,” said Tiernan.
GB&I have not won the Walker Cup since 2015 and are without a win on American soil since 2001.
The next edition of the tournament will be in 2026 at Lahinch Golf Club in County Clare, Republic of Ireland.
The competition usually takes place every two years but is shifting to even-numbered years to avoid conflicting with the World Amateur Team Championships.